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Microbiology

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Sputum

Examination of sputum has a low predictive value for clinical decision-making, except for Mycobacteria spp, Nocardia spp and Legionella spp.

However, there may be circumstances where a result is beneficial, such as in Cystic Fibrosis, Bronchiectasis, COPD, Chest Unit patients, those under chest physicians and immunocompromised patients.

Note: where Pseudomonas aeruginosa is isolated from Cystic Fibrosis and Bronchiectasis patients we apply sensitivity testing criteria (with the exception of isolates from GP patients).

Sensitivity testing will only be performed in the following cases.

  • It is the first isolate.
  • It is more than 12 months since the last isolate was tested.
  • If the clinical details mention poor response to therapy, commencing therapy or there is a specific request for sensitivity testing.

Sample type

  • Sputum, Endotracheal or Tracheal Aspirates, Nasopharyngeal Aspirates / Secretions
  • Cough Swabs
  • Antral / Sinus Washouts
  • Endotracheal Tips

Specimen requirements

Send in a sterile universal container. For cough swabs use a ∑-TRANSWAB® (MWE) tube (purple top)

For sputum samples - ensure specimen comprises sputum (not saliva / food); samples of saliva only will not be processed

Minimum volume

For routine culture - 1ml

For further tests - 5ml

Frequency of test

Daily

Turnaround time

Routine respiratory culture - 5 days

What to request on EPR

Respiratory MCS - > specimen type sputum

Last reviewed:16 February 2023